gos antagonistic activity against e coli atcc 8739 (ATCC)

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ATCC gos antagonistic activity against e coli atcc 8739

Schematic representation of the experimental design. The models and the methods used are indicated, with references to the corresponding paragraph numbers of the Materials and Methods section. ETEC F4 + , enterotoxigenic <t>Escherichia</t> <t>coli</t> F4 + ; L. amylovorus ATCC 33198, Lactobacillus amylovorus ATCC 33198; L. amylovorus DSM 16698, Lactobacillus amylovorus DSM 16698; L. reuteri DSM 110571, Limosilactobacillus reuteri subsp. porcinus DSM 110571; Papp, apparent permeability; P-p65, phosphorylated p65 protein; TEER, transepithelial electrical resistance; TJ, tight junction.

Gos Antagonistic Activity Against E Coli Atcc 8739, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 6585 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gos antagonistic activity against e coli atcc 8739 - by Bioz Stars, 2026-10

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1) Product Images from "Protective activity of galacto-oligosaccharides against intestinal damage and inflammation induced by enterotoxigenic Escherichia coli F4 + and evaluation of prebiotic potential"

Article Title: Protective activity of galacto-oligosaccharides against intestinal damage and inflammation induced by enterotoxigenic Escherichia coli F4 + and evaluation of prebiotic potential

Journal: Frontiers in Veterinary Science

doi: 10.3389/fvets.2025.1740099

Figure Legend Snippet: Schematic representation of the experimental design. The models and the methods used are indicated, with references to the corresponding paragraph numbers of the Materials and Methods section. ETEC F4 + , enterotoxigenic Escherichia coli F4 + ; L. amylovorus ATCC 33198, Lactobacillus amylovorus ATCC 33198; L. amylovorus DSM 16698, Lactobacillus amylovorus DSM 16698; L. reuteri DSM 110571, Limosilactobacillus reuteri subsp. porcinus DSM 110571; Papp, apparent permeability; P-p65, phosphorylated p65 protein; TEER, transepithelial electrical resistance; TJ, tight junction.

Techniques Used: Permeability

Reduction of enterotoxigenic Escherichia coli (ETEC) F4 + adhesion (A) and invasion (B) in differentiated Caco-2 cells mediated by GOS. ETEC F4 + was allowed to adhere or invade Caco-2 cells, either alone (ETEC) or in the presence of 2% GOS (ETEC + GOS). Data are reported as log colony forming units (CFU)/ml recovered after plating. Columns represent the mean ± SD of at least two independent experiments, each performed in technical duplicate. Statistical analysis was performed by Student's t -test (* P < 0.001 and P < 0.01 for adhesion and invasion assays, respectively).

Figure Legend Snippet: Reduction of enterotoxigenic Escherichia coli (ETEC) F4 + adhesion (A) and invasion (B) in differentiated Caco-2 cells mediated by GOS. ETEC F4 + was allowed to adhere or invade Caco-2 cells, either alone (ETEC) or in the presence of 2% GOS (ETEC + GOS). Data are reported as log colony forming units (CFU)/ml recovered after plating. Columns represent the mean ± SD of at least two independent experiments, each performed in technical duplicate. Statistical analysis was performed by Student's t -test (* P < 0.001 and P < 0.01 for adhesion and invasion assays, respectively).

Techniques Used:

GOS effects on enterotoxigenic Escherichia coli (ETEC) F4 + -induced permeability increase in Caco-2 cells: Transepithelial electrical resistance (TEER, A ) and phenol red apparent permeability (Papp, B ). Cells, differentiated on permeable filters, were untreated (Control, C), infected with ETEC F4 + , or treated with 2% GOS, either alone or in combination with ETEC. TEER values were recorded every 30 min for up to 150 min. The TEER values of treated cells were expressed as % of the TEER value of control filters, for each time point. Phenol red Papp was measured at 150 min, and values are reported as cm s −1 . A red line set at 1 × 10 −6 cm s −1 represents Papp threshold, indicating destroyed cell monolayer integrity for values above. Values represent means ± SD of three independent experiments, carried out at least in duplicate. Statistical analysis was performed only at final time points (2.5 h). Means without a common letter significantly differ, P < 0.001.

Figure Legend Snippet: GOS effects on enterotoxigenic Escherichia coli (ETEC) F4 + -induced permeability increase in Caco-2 cells: Transepithelial electrical resistance (TEER, A ) and phenol red apparent permeability (Papp, B ). Cells, differentiated on permeable filters, were untreated (Control, C), infected with ETEC F4 + , or treated with 2% GOS, either alone or in combination with ETEC. TEER values were recorded every 30 min for up to 150 min. The TEER values of treated cells were expressed as % of the TEER value of control filters, for each time point. Phenol red Papp was measured at 150 min, and values are reported as cm s −1 . A red line set at 1 × 10 −6 cm s −1 represents Papp threshold, indicating destroyed cell monolayer integrity for values above. Values represent means ± SD of three independent experiments, carried out at least in duplicate. Statistical analysis was performed only at final time points (2.5 h). Means without a common letter significantly differ, P < 0.001.

Techniques Used: Permeability, Control, Infection

GOS effects on enterotoxigenic Escherichia coli (ETEC) F4 + -induced occludin delocalization in Caco-2 cells, differentiated on permeable filters, assayed by immunofluorescence. Cells were untreated (Control, C), infected with ETEC F4 + , or treated with 2% GOS, either alone or in combination with ETEC. Cells were then labeled with specific primary antibody for occludin, followed by TRITC-conjugated secondary antibody and 4′,6-diamidino-2-phenylindole (DAPI). In the figure the separate stainings and the merge of them are shown. Interruption of continuous staining of occludin, resulting in dissociation of the protein from membranes in ETEC F4 + infected cells is visible (white arrows), as well as regular localization in control cells or treated with GOS either alone or in combination with ETEC F4 + . Each figure is representative of three independent immunofluorescence assays (40 × magnification). Bars represent 10 μm.

Figure Legend Snippet: GOS effects on enterotoxigenic Escherichia coli (ETEC) F4 + -induced occludin delocalization in Caco-2 cells, differentiated on permeable filters, assayed by immunofluorescence. Cells were untreated (Control, C), infected with ETEC F4 + , or treated with 2% GOS, either alone or in combination with ETEC. Cells were then labeled with specific primary antibody for occludin, followed by TRITC-conjugated secondary antibody and 4′,6-diamidino-2-phenylindole (DAPI). In the figure the separate stainings and the merge of them are shown. Interruption of continuous staining of occludin, resulting in dissociation of the protein from membranes in ETEC F4 + infected cells is visible (white arrows), as well as regular localization in control cells or treated with GOS either alone or in combination with ETEC F4 + . Each figure is representative of three independent immunofluorescence assays (40 × magnification). Bars represent 10 μm.

Techniques Used: Immunofluorescence, Control, Infection, Labeling, Staining

GOS effects on enterotoxigenic Escherichia coli (ETEC) F4 + -induced ZO-1 delocalization in Caco-2 cells, differentiated on permeable filters, assayed by immunofluorescence. Cells were untreated (Control, C), infected with ETEC F4 + , or treated with 2% GOS, either alone or in combination with ETEC. Cells were then labeled with specific primary antibody for ZO-1, followed by TRITC-conjugated secondary antibody and 4′,6-diamidino-2-phenylindole (DAPI). In the figure the separate stainings and the merge of them are shown. Interruption of continuous staining of ZO-1, resulting in dissociation of the protein from membranes in ETEC F4 + infected cells is visible (white arrows), as well as regular localization in control cells or treated with GOS either alone or in combination with ETEC F4 + . Each figure is representative of three independent immunofluorescence assays (40 × magnification). Bars represent 10 μm.

Figure Legend Snippet: GOS effects on enterotoxigenic Escherichia coli (ETEC) F4 + -induced ZO-1 delocalization in Caco-2 cells, differentiated on permeable filters, assayed by immunofluorescence. Cells were untreated (Control, C), infected with ETEC F4 + , or treated with 2% GOS, either alone or in combination with ETEC. Cells were then labeled with specific primary antibody for ZO-1, followed by TRITC-conjugated secondary antibody and 4′,6-diamidino-2-phenylindole (DAPI). In the figure the separate stainings and the merge of them are shown. Interruption of continuous staining of ZO-1, resulting in dissociation of the protein from membranes in ETEC F4 + infected cells is visible (white arrows), as well as regular localization in control cells or treated with GOS either alone or in combination with ETEC F4 + . Each figure is representative of three independent immunofluorescence assays (40 × magnification). Bars represent 10 μm.

Techniques Used: Immunofluorescence, Control, Infection, Labeling, Staining

$GOS effects on enterotoxigenic Escherichia coli (ETEC) F4 + -induced NF-kB activation in differentiated Caco-2 cells, assayed by Western blotting. Cells were untreated (Control, C), infected with ETEC F4 + , or treated with 2% GOS, either alone or in combination with ETEC. Cell lysates were fractionated by SDS-PAGE and transferred to nitrocellulose filters. Membranes were incubated with rabbit polyclonal anti-p65 or anti-phosphorylated (P) -p65 primary antibodies and then with horseradish peroxidase-conjugated secondary antibodies. (A) densitometric values of P-p65 protein, normalized to its corresponding unphosphorylated form, after verifying equal protein loading with α-tubulin, used as internal control. Values represent means ± SD of three independent experiments, carried out in triplicate. Means without a common letter significantly differ ( P < 0.05). (B) representative gel.$
Figure Legend Snippet: GOS effects on enterotoxigenic Escherichia coli (ETEC) F4 + -induced NF-kB activation in differentiated Caco-2 cells, assayed by Western blotting. Cells were untreated (Control, C), infected with ETEC F4 + , or treated with 2% GOS, either alone or in combination with ETEC. Cell lysates were fractionated by SDS-PAGE and transferred to nitrocellulose filters. Membranes were incubated with rabbit polyclonal anti-p65 or anti-phosphorylated (P) -p65 primary antibodies and then with horseradish peroxidase-conjugated secondary antibodies. (A) densitometric values of P-p65 protein, normalized to its corresponding unphosphorylated form, after verifying equal protein loading with α-tubulin, used as internal control. Values represent means ± SD of three independent experiments, carried out in triplicate. Means without a common letter significantly differ ( P < 0.05). (B) representative gel.

Techniques Used: Activation Assay, Western Blot, Control, Infection, SDS Page, Incubation

GOS effects on enterotoxigenic Escherichia coli (ETEC) F4 + -induced P-p65 translocation into the nucleus in Caco-2 cells, differentiated on permeable filters, assayed by immunofluorescence. Cells were untreated (Control), infected with ETEC F4 + , or treated with 2% GOS, either alone or in combination with ETEC. Cells were then labeled with specific primary antibody for P-p65, followed by TRITC-conjugated secondary antibody and 4′,6-diamidino-2-phenylindole (DAPI). In the figure the separate stainings and the merge of them are shown. Each figure is representative of three independent immunofluorescence assays (40 × magnification). Bars represent 10 μm.

Figure Legend Snippet: GOS effects on enterotoxigenic Escherichia coli (ETEC) F4 + -induced P-p65 translocation into the nucleus in Caco-2 cells, differentiated on permeable filters, assayed by immunofluorescence. Cells were untreated (Control), infected with ETEC F4 + , or treated with 2% GOS, either alone or in combination with ETEC. Cells were then labeled with specific primary antibody for P-p65, followed by TRITC-conjugated secondary antibody and 4′,6-diamidino-2-phenylindole (DAPI). In the figure the separate stainings and the merge of them are shown. Each figure is representative of three independent immunofluorescence assays (40 × magnification). Bars represent 10 μm.

Techniques Used: Translocation Assay, Immunofluorescence, Control, Infection, Labeling

Activity Assay:

Article Title: Bacillus subtilis EGY1 glucansucrase: optimization, characterization and immobilization using activated carrier of pectin-egg white protein beads
Article Snippet: .. The antimicrobial activity indicated that all of the examined samples possessed varied activity against Staphylococcus aureus ATCC 6538 and Candida albicans ATCC 10231 without the estimation of any activity against Escherichia coli ATCC 8739 (Fig. , Table ). ..

Article Title: Traditional Applications, Phytochemical Constituents, and Pharmacological Properties of Lavandula multifida L.: A Review.
Article Snippet: .. Tofah et al. [43] evaluated its activity against Escherichia coli ATCC 8739 and Staphylococcus aureus ATCC 6583 using the agar well diffusion method, revealing notable efficacy (MIC = 0.625 μg/mL) with greater sensitivity in E. coli (inhibition diameter = 22.6 mm) compared to S. aureus (17.8 mm). ..

Article Title: Bacillus subtilis EGY1 glucansucrase: optimization, characterization and immobilization using activated carrier of pectin-egg white protein beads.
Article Snippet: .. The antimicrobial activity indicated that all of the examined samples possessed varied activity against Staphylococcus aureus ATCC 6538 and Candida albicans ATCC 10231 without the estimation of any activity against Escherichia coli ATCC 8739 (Fig. 2, Table 3). ..

Article Title: Traditional Applications, Phytochemical Constituents, and Pharmacological Properties of Lavandula multifida L.: A Review
Article Snippet: .. Tofah et al. [ ] evaluated its activity against Escherichia coli ATCC 8739 and Staphylococcus aureus ATCC 6583 using the agar well diffusion method, revealing notable efficacy (MIC = 0.625 μg/mL) with greater sensitivity in E. coli (inhibition diameter = 22.6 mm) compared to S. aureus (17.8 mm). ..

Article Title: Traditional Applications, Phytochemical Constituents, and Pharmacological Properties of Lavandula multifida L.: A Review.
Article Snippet: .. They evaluated its activity against Escherichia coli ATCC 8739 and Staphylococcus aureus ATCC 6583 using the agar well diffusion method, revealing notable efficacy (MIC = 0.625 μg/mL) with greater sensitivity in E. coli (inhibition diameter = 22.6 mm) compared to S. aureus (17.8 mm). ..

Article Title: Enumeration and antimicrobial activity of bacterial isolates from undisturbed and contaminated soils in North Macedonia
Article Snippet: Particularly in temperate regions, seasonal change has an effect on microbial communities directly through climatic function.. The microbial populations of an undisturbed and contaminated soil undergo temporal change over the course of four seasons.. Eight soil samples were collected from different regions in North Macedonia.

Article Title: Traditional Applications, Phytochemical Constituents, and Pharmacological Properties of Lavandula multifida L.: A Review
Article Snippet: .. They evaluated its activity against Escherichia coli ATCC 8739 and Staphylococcus aureus ATCC 6583 using the agar well diffusion method, revealing notable efficacy (MIC = 0.625 μg/mL) with greater sensitivity in E. coli (inhibition diameter = 22.6 mm) compared to S. aureus (17.8 mm). ..

Diffusion-based Assay:

Inhibition:

other:

Article Title: Chemical constituents of Palisota ambigua (Commelinaceae) with their antibacterial activities
Article Snippet: Background: The development and spread of resistance by bacteria to existing antibiotics are becoming more common nowadays.. Considering the ethnopharmacological relevance of Palisota ambigua (Commelinaceae), this study was designed to investigate the antibacterial activities of chemical constituents of P. ambigua against two Gram-positive (Staphylococcus aureus ATCC 25923 and Streptococcus pneumoniae ATCC 49619) and two Gram-negative (Escherichia coli ATCC 8739 and Klebsiella pneumoniae 109) bacteria.. Methods: The plant extracts were prepared by maceration in organic solvents.

Review